Guinness World Record confirmed to the BBC that the festive spectacle at Sydney's Bondi Beach on Tuesday exceeded the target of 250 surfers.
The organisers say the record-breaking surf is also an attempt to raise awareness of mental health issues.
The event was kick-started by a local retailer, surf school and surf charity.
RedBalloon, Let's Go Surfing, and OneWave came together to hold the lesson, and rustled up hundreds of participants using social media.
OneWave spokesperson and mental health therapist, Joel Pilgrim told the BBC the surf charity started about three years as a way to bring the local community together and to break down the stigma surrounding mental health issues.